Yoshikiyo Research Group

YOSHIKIYO
Keisuke

吉清 恵介
Professor
Department of Life Sciences
Faculty of Life and Environmental Sciences
Shimane University

Cyclodextrin Group
シクロデキストリングループ

The science of healthy foods
using edible molecular capsules

30+
Papers
18+
Years
3
Societies

ResearchYoshikiyo Research Group

Our research uses cyclodextrins, cyclic oligosaccharides with a hydrophobic cavity that can selectively capture various molecules and ions — a phenomenon known as "inclusion." Because cyclodextrins are themselves edible, we use this "edible molecular capsule" as a platform for applied food chemistry research.

At the core of our work is cyclodextrin, the "edible molecular capsule." By encapsulating health-promoting components such as perilla (egoma) oil, we can turn them into food materials that are absorbed more efficiently, resist oxidation, and are easier to handle. Our goal is to solve, at the molecular level, the common problem that "things known to be healthy are hard to keep up with."

🫙

Turning oil into a powder

We succeeded in converting liquid perilla oil into a free-flowing powder, making it more resistant to oxidation and easier to add to food.

Improved handling
💊

Improved omega-3 absorption

Co-ingestion with cyclodextrin was shown in rat studies to significantly increase blood omega-3 concentrations.

Better delivery to the body
🫀

Effects on organ fatty acid balance

We found that omega-3 fatty acid composition changes not only in blood but also in organs such as the liver and brain.

Evidence for health benefits
🔬

Safe and practical, because it's edible

Cyclodextrin is an approved food additive. We pursue this work as "food science," not as pharmaceutical development.

Food chemistry / applied research
THEME 01

Inclusion Chemistry of Cyclodextrins

We have studied inclusion complex formation between α-, β-, and γ-cyclodextrins and a variety of guest molecules, using NMR spectroscopy and thermodynamic analysis to understand the mechanisms and selectivity of molecular recognition.
Details →

THEME 02

Encapsulation of Perilla Oil

We prepared inclusion complexes of γ-cyclodextrin with perilla oil, successfully producing a powdered form with improved resistance to oxidation and greater thermal stability — making it much easier to handle as a food ingredient.
Details →

THEME 03

Enhanced Bioavailability of α-Linolenic Acid

Rat and mouse studies show that ingestion as a γ-CD inclusion complex significantly raises plasma α-linolenic acid levels. We are working to elucidate the underlying mechanism.
Details →

THEME 04

Effect on Organ Fatty Acid Composition

Ingesting perilla oil encapsulated in γ-cyclodextrin (γ-CD) was found to affect fatty acid composition not only in the blood but in various organs as well.
Details →

ProfileCurriculum Vitae

As a child, I was fascinated by how things worked and often took apart appliances and machines. Later I became interested in agriculture and chemistry, helping out on my grandfather's mandarin orange farm. As a university student, I had the opportunity to work on organic chemistry (synthesis of cyclodextrin derivatives) and physical chemistry (mainly the second law of thermodynamics). Building on that background, I now work on applied research in food chemistry.
Degree Ph.D. (Agriculture) — Tottori University (March 2008)
Societies Cyclodextrin Society of Japan / Japanese Society of Nutrition and Food Science / The Chemical Society of Japan
Contact yoshikiyo [at] life.shimane-u.ac.jp

Education

2005–2008
United Graduate School of Agricultural Sciences, Tottori University
2002–2004
Graduate School of Life and Environmental Sciences, Shimane University
1998–2002
Faculty of Life and Environmental Sciences, Shimane University
1995–1998
Hiroshima Kogyo University Senior High School
(now Hiroshima Nagisa Senior High School)

Career

2025–present
Professor, Shimane University
2018–2025
Associate Professor, Shimane University
2008–2018
Assistant Professor, Shimane University

PublicationsPeer-Reviewed Articles

2026 3 papers
Keisuke Yoshikiyo
Encapsulation of Perilla Oil with Cyclodextrin: Improving Powder Form and Digestibility/Absorption (in Japanese)
Surface and Interface (Review), Vol. 63, No. 5, p.181-186 (May 10, 2026)
doi:10.4164/sptj.63.181
Mohammad Ariful Islam Bhuiya, Keisuke Yoshikiyo, Yukine Moda, Pinky Karim Syeda K Fatima, Kaeko Murota
Functional evaluation of perilla leaf tea as a pancreatic lipase inhibitor: roles of rosmarinic acid and emulsion structure
Bioscience, Biotechnology, and Biochemistry
doi:10.1093/bbb/zbag021
Hideaki Kawahara, Yoshihito Koto, Yuuka Hitsuda, Koichi Kurata, Keisuke Yoshikiyo, et al.
Microcystin-LR Drives Early NAFLD Pathogenesis via Hepatic Cholesterol Accumulation: Dysregulation of Ldlr and Abcg1 Expression Uncoupled from Srebp2
Toxins
doi:10.3390/toxins18020092
2025 5 papers
Ayame Tomii, Chihiro Takei, Keisuke Yoshikiyo, Hidehisa Shimizu
The Gut Microbial Metabolite Indole-3-Acetic Acid Reprograms Systemic Homeostasis and Ameliorates IBD-Associated Cachexia Independent of Food Intake
International Journal of Molecular Sciences, 26, 11260 (2025)
doi:10.3390/ijms262311260
Noritaka Hirohashi, Yoshiaki Tanaka, Seiji Sasai, Tomohiro Sasanami, Hiroki Ono, Yoko Iwata, Eiji Fujiwara, Keisuke Yoshikiyo, Miwa Tamura-Nakano
Discovery of adipocyte-like accessory reproductive cells in male giant squid
microPublication Biology
doi:10.17912/micropub.biology.001494
Katsunori Ishii, Yu Ichisaka, Hikaru Sugiyama, Rin Kobayashi, Fuzuki Sakamoto, Kazuhisa Watanabe, Keisuke Yoshikiyo, Hidehisa Shimizu
Hexaraphane Affects the Activation of Hepatic PPARα Signaling: Impact on Plasma Triglyceride Levels and Hepatic Senescence with Aging
Nutrients
doi:10.3390/nu17111768
Yoshikiyo, K.; Shimizu, H.; Nagato, E.G.; Ishizuka, S.; Yamamoto, T.
Comparative Analysis of γ-Cyclodextrin, Perilla Oil, and Their Inclusion Complexes on Liver Injury and Dyslipidemia Associated with Elevated Gastrointestinal 12-Hydroxylated Bile Acid Levels
Molecules
doi:10.3390/molecules30020281
Yoshikiyo, K.; Shimizu, H.; Okada, H.; Hasegawa, A.; Yamamoto, T.
γ-Cyclodextrin Co-Ingestion Enhances the Bioavailability of Perilla Oil, Regardless of Inclusion Complex Formation
International Journal of Molecular Sciences, 26 (2025)
doi:10.3390/ijms26167776
2024 3 papers
Hitsuda, Y.; Koto, Y.; Kawahara, H.; Kurata, K.; Yoshikiyo, K.; et al.
Increased Prorenin Expression in the Kidneys May Be Involved in the Abnormal Renal Function Caused by Prolonged Environmental Exposure to Microcystin-LR
Toxics
doi:10.3390/toxics12080547
Ino, M.; Kihara, J.; Ishihara, A.; Seki, K.; Tanaka, T.; Yoshikiyo, K.; Ueno, M.
Identification of antifungal compound isolated from Cercospora sp. ME202 and evaluation of its inhibitory activity against Colletotrichum orbiculare
Journal of General Plant Pathology
doi:10.1007/s10327-023-01153-1
Honda, M.; Nakatsuka, A.; Esumi, T.; Yamauchi, N.; Yoshikiyo, K.; et al.
Enzymatic tissue browning caused by biological membrane degradation in the young immature fruit of 'Moriya' persimmon
Scientia Horticulturae
doi:10.1016/j.scienta.2023.112555
2023 1 paper
Yoshikiyo, K.; Takahashi, M.; Narumiya, Y.; Honda, M.; Iwasaki, K.; Ishigaki, M.; Nagato, E.G.; Noothalapati, H.; Shimizu, H.; Murota, K.; Yamamoto, T.
Co-ingestion with γ-cyclodextrin improves bioavailability of α-linolenic acid in Perilla frutescens seed oil
Food Hydrocolloids for Health
doi:10.1016/j.fhfh.2023.100116
2022 2 papers
Akihiro, T.; Yasui, R.; Yasuhira, S.; Matsumoto, K.I.; Tanaka, Y.; Matsuo, Y.; Shimizu, H.; Matsuzaki, T.; Matsumoto, S.; Yoshikiyo, K.; Ishida, H.
Tropomyosin micelles are the major components contributing to the white colour of boiled shellfish soups
Scientific Reports
doi:10.1038/s41598-022-17911-8
Ishigaki, M.; Ito, A.; Hara, R.; Miyazaki, S.I.; Murayama, K.; Tsuji, S.; Inomata, M.; Yoshikiyo, K.; Yamamoto, T.; Ozaki, Y.
Development of an amino acid sequence-dependent analytical method for peptides using near-infrared spectroscopy
Analyst
doi:10.1039/d2an00895e
2021–2007 earlier works
Ishigaki, M.; et al.; Yoshikiyo, K.; Yamamoto, T.; Ozaki, Y.
Method of Monitoring the Number of Amide Bonds in Peptides Using Near-Infrared Spectroscopy (2021)
Analytical Chemistry
doi:10.1021/acs.analchem.0c03424
Keisuke Yoshikiyo
Expanding Applications through Powdered Perilla Oil (2020) (in Japanese)
Journal of Industry-Academia-Government Collaboration
doi:10.1241/sangakukanjournal.16.11_4
Yoshikiyo, K.; Yoshioka, Y.; Narumiya, Y.; Oe, S.; Kawahara, H.; Kurata, K.; Shimizu, H.; Yamamoto, T.
Thermal stability and bioavailability of inclusion complexes of perilla oil with γ-cyclodextrin (2019)
Food Chemistry
doi:10.1016/j.foodchem.2019.04.093
Papers from 2007–2018 are listed in full on researchmap.
→ researchmap.jp/read0142537

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Office
Room 313, Building 1, Faculty of Life and Environmental Sciences
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Affiliation
Department of Life Sciences
Faculty of Life and Environmental Sciences, Shimane University
Address
1060 Nishikawatsu-cho, Matsue
Shimane 690-8504, Japan
TEL/FAX
+81-852-32-6571
Email
yoshikiyo [at] life.shimane-u.ac.jp
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